Applied and Environmental Microbiology · 1997 · 63 citations · 10 references
AgricultureFood ScienceMicrobiologyBacteriologyDairy MicrobiologyFood MicrobiologyFoodborne Pathogen DetectionMicrobial TranscriptomicsNucleic Acid Amplification TestingNucleic Acid ExtractionPathogen Detection In FoodsPolymerase Chain ReactionAmplification SpecificityEnrichment BrothListeria DetectionNucleic Acid AmplificationNucleic Acid Amplification DiagnosticsNucleic Acid Sequence-based AmplificationPathogen Detection By AmplificationPathogen Nucleic Acid Detection
A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.
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