Concepedia

Validation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in strawberry fruits using different cultivars and osmotic stresses

Vanessa Galli, Joyce Moura Borowski, Ellen Cristina Perin, Rafael da Silva Messias, Julia Labonde, Ivan dos Santos Pereira, Sérgio Delmar dos Anjos e Silva, César Valmor Rombaldi

Gene · 2014 · 104 citations · 45 references

DOI

Concepts

References

45

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 176.9K citations

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 19.7K citations · Full text

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Stephen A. Bustin, Vladimı́r Beneš, Jeremy A. Garson et al. · Clinical Chemistry · 2009 · 15.6K citations · Full text

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004 · 7.1K citations

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Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5K citations

, , +13