An efficient protein complex purification method for functional proteomics in higher eukaryotes

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

John David Dignam, Russell M. Lebovitz, R G Roeder · Nucleic Acids Research · 1983 · 11.6K citations · Full text

Engineering, Rna Polymerase Ii, Isolated Mammalian Nuclei +19

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

Andrej Shevchenko, Matthias Wilm, Ole Vorm et al. · Analytical Chemistry · 1996 · 9K citations

Functional organization of the yeast proteome by systematic analysis of protein complexes

Anne‐Claude Gavin, Markus Bösche, Roland Krause et al. · Nature · 2002 · 4.8K citations · Full text

Yeast Proteome, Biochemistry, Protein Folding +10

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry

Yuen Ho, Albrecht Gruhler, Adrian Heilbut et al. · Nature · 2002 · 3.6K citations

Systematic Identification, Biochemistry, Interactomics +12

A generic protein purification method for protein complex characterization and proteome exploration

Guillaume Rigaut, Anna Shevchenko, Berthold Rutz et al. · Nature Biotechnology · 1999 · 2.7K citations

Proteomic Technology, Biochemistry, Protein Folding +10