A Rapid Method for Protein Extraction from Fission Yeast

Yuzy Matsuo, Kazuhide Asakawa, Takashi Toda, Satoshi Katayama

Bioscience Biotechnology and Biochemistry · 2006 · 96 citations · 2 references

DOIFull text

Open access

Concepts

Abstract

Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.

References

2