Bioscience Biotechnology and Biochemistry · 2006 · 96 citations · 2 references
Life SciencesBiochemistryCell BiologyBioseparationsProteomicsSeparation ProcessesBottom-up ProteomicsExtraction TechniquesProtein ElectrophoresisProtein PurificationProtein SeparationProtein ExtractionProtein RecoveryProtein Sample PreparationProtein Separation TechniquesSample Preparation For Intact ProteinsWestern Blotting
Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.
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Rapid and reliable protein extraction from yeast
Vitaly V. Kushnirov · Yeast · 2000 · 1K citations
Ryoichi Saiki, Ai Nagata, Naonori Uchida et al. · European Journal of Biochemistry · 2003 · 59 citations · Full text