Proteolytic enzyme-immobilization techniques for MS-based protein analysis

Organic−Inorganic Hybrid Silica Monolith Based Immobilized Trypsin Reactor with High Enzymatic Activity

Junfeng Ma, Zhen Liang, Xiaoqiang Qiao et al. · Analytical Chemistry · 2008 · 200 citations

Life Sciences, Biochemistry, Biotechnology +17

Dual-Function Microanalytical Device by In Situ Photolithographic Grafting of Porous Polymer Monolith:  Integrating Solid-Phase Extraction and Enzymatic Digestion for Peptide Mass Mapping

Dominic S. Peterson, Thomas Rohr, František Švec et al. · Analytical Chemistry · 2003 · 185 citations

Engineering, Bioengineering, Biotechnology +17

Highly Efficient Enzyme Reactors Containing Trypsin and Endoproteinase LysC Immobilized on Porous Polymer Monolith Coupled to MS Suitable for Analysis of Antibodies

Jana Křenková, Nathan A. Lacher, František Švec · Analytical Chemistry · 2009 · 167 citations

Life Sciences, Biochemistry, Biotechnology +13

High-Throughput Peptide Mass Mapping Using a Microdevice Containing Trypsin Immobilized on a Porous Polymer Monolith Coupled to MALDI TOF and ESI TOF Mass Spectrometers

Dominic S. Peterson, Thomas Rohr, František Švec et al. · Journal of Proteome Research · 2002 · 139 citations

Life Sciences, Biochemistry, Biotechnology +17

Multilayer-Assembled Microchip for Enzyme Immobilization as Reactor Toward Low-Level Protein Identification

Yun Liu, Haojie Lu, Wei Zhong et al. · Analytical Chemistry · 2005 · 130 citations · Full text

Life Sciences, Biochemistry, Biotechnology +17