A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

A simple method for displaying the hydropathic character of a protein

Jack Kyte, Russell F. Doolittle · Journal of Molecular Biology · 1982 · 22.9K citations

Protein Chemistry, Biochemistry, Simple Method +12

Improved tools for biological sequence comparison.

William R. Pearson, David J. Lipman · Proceedings of the National Academy of Sciences · 1988 · 11.3K citations · Full text

Genetics, Molecular Biology, Dna Sequences +18

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

W. Neal Burnette · Analytical Biochemistry · 1981 · 8.5K citations

Biochemistry, Natural Sciences, Bioanalysis +10

Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

Cell Division, Natural Sciences, Biotechnology +15