Concepedia

Genomic DNA Amplification from a Single Bacterium

Arumugham Raghunathan, Harley R. Ferguson, Carole J. Bornarth, Wanmin Song, Mark D. Driscoll, Roger S. Lasken

Applied and Environmental Microbiology · 2005 · 305 citations · 6 references

DOIFull text

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Concepts

Abstract

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

References

6

Comprehensive human genome amplification using multiple displacement amplification

Frank B. Dean, Seiyu Hosono, Linhua Fang et al. · Proceedings of the National Academy of Sciences · 2002 · 1.5K citations

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Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

Frank B. Dean, John Nelson, Theresa L. Giesler et al. · Genome Research · 2001 · 1.1K citations · Full text

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Unbiased Whole-Genome Amplification Directly From Clinical Samples

Seiyu Hosono, A. Fawad Faruqi, Frank B. Dean et al. · Genome Research · 2003 · 420 citations · Full text

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Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array–CGH

José M. Lage, John H. Leamon, Tanja Pejović et al. · Genome Research · 2003 · 265 citations · Full text

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Isothermal whole genome amplification from single and small numbers of cells: a new era for preimplantation genetic diagnosis of inherited disease

Alan H. Handyside, Mark D. Robinson, Robert J. Simpson et al. · Molecular Human Reproduction · 2004 · 244 citations · Full text

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