Low irradiance alters carbon metabolism and delays flower stalk development in two orchids

Abstract

In Phalaenopsis, lowering irradiance has been used to delay flower stalk development but the accompanying biochemical changes remain poorly understood. We cultured two commercial Phalaenopsis-type orchids, Phalaenopsis cv. Sogo Yukidian V3, and Doritaneopsis cv. Walnut Valley Halo ES09 under reduced irradiance by under-bench shading (approximately 15 % of mean control irradiance) for 15 weeks in a greenhouse under the natural photoperiod. Besides delaying flower stalk development as expected, the treatment greatly decreased the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase, phosphoenolpyruvate carboxylase, and NAD+-malic enzyme, and reduced the nocturnal malate accumulation and daytime starch deposition, the typical diurnal metabolite fluctuations of crassulacean acid metabolism (CAM) plants. As well, the content of sucrose and starch was reduced at dawn and dusk whereas the content of glucose and fructose only at dawn. The persistent decrease in the sucrose content under shading may be an inhibitory signal of flower stalk induction.