Hydrolysis of Lactose by Microbial β-Galactosidases. Formation of Oligosaccharides with Special Reference to 2-0-β-D-galactopyranosyl-D-glucose

Abstract

The transgalactosylation of lactose by Saccharomyces fragilis 3-galactosidase and Aspergillus niger /3-galactosidase was examined. With S. fragilis /3-galactosidase, twelve oligosaccharides including five disaccharides were detected on paper chromatograms. With A. niger/3-galactosidase, ten oligosaccharides were formed. The disaccharides formed by A. niger/3-galactosidase were similar to those by S.