Concepedia

Cysteine-Derived <i>S</i>-Protected Oxazolidinones:  Potential Chemical Devices for the Preparation of Peptide Thioesters

Yusuke Ohta, S Itoh, Akira Shigenaga, Saori Shintaku, Nobutaka Fujii, Akira Otaka

Organic Letters · 2006 · 96 citations · 14 references

DOI

Concepts

Abstract

[reaction: see text]. An N-S acyl-transfer-mediated preparation of peptide thioesters using the S-protected oxazolidinone derived from cysteine has been developed and applied to the synthesis of a 32-mer biologically active peptide by native chemical ligation protocols.

References

14

Synthesis of Proteins by Native Chemical Ligation

Philip E. Dawson, Tom W. Muir, Ian Clark‐Lewis et al. · Science · 1994 · 4.1K citations

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

Shaorong Chong, Fana B. Mersha, Donald G. Comb et al. · Gene · 1997 · 606 citations

, , +16

A Theropod Dinosaur Embryo and the Affinities of the Flaming Cliffs Dinosaur Eggs

Mark A. Norell, James M. Clark, Barsbold Rhinchen et al. · Science · 1994 · 326 citations

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Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

Shaorong Chong, G. E. Montello, A. Zhang et al. · Nucleic Acids Research · 1998 · 217 citations

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Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein–intein junction

Alessandra Romanelli, Alexander Shekhtman, David Cowburn et al. · Proceedings of the National Academy of Sciences · 2004 · 164 citations · Full text

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