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Synthesis of Sialyllactose from<i>N</i>-Acetylneuraminic Acid and Lactose by a Neuraminidase from<i>Arthrobacter ureafaciens</i>
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1992
Year
Bioorganic ChemistryGlycobiologyPolysaccharideCarbohydrate-protein InteractionEnzymatic ModificationBiosynthesisBioanalysisNatural Product BiosynthesisBiotransformationBiochemistryBiocatalysisSialyllactose IsomersBiomolecular EngineeringIon-exchange ChromatographyNatural SciencesEnzyme CatalysisMass SpectrometryMicrobiologyMedicineSynthetic Chemistry
Two sialyllactose isomers, NeuAcα2→6Galβ1→4Glc and Galβ1→4(NeuAcα2→6)Glc, were prepared by incubation of a concentrated solution of N-acetylneuraminic acid and lactose in the presence of a neuraminidase from Arthrobacter ureafaciens. Each sialyllactose was isolated by a combination of ion-exchange chromatography and high performance liquid chromatography. The structure of each sialyllactose was identified by mass spectrometry, nuclear magnetic resonance spectrometry, and enzymatic analysis.