Requirement of the carboxy‐terminal domain of RNA polymerase II for the transcriptional activation of chromosomal <i>c‐fos</i> and <i>hsp</i>70A genes

Abstract

The carboxy-terminal domain of the large subunit of mouse and human RNA polymerase II contains 52 repeats of a heptapeptide which are the targets for a variety of kinases. We have used an alpha-amanitin resistant form of the large subunit of pol II to study the role of the carboxy-terminal domain in the expression of chromosomal genes. The large subunit of RNA polymerase II and deletion mutants thereof, which contain only 31 (LSdelta31) and 5 (LSdeltaS) repeats, were expressed in 293 cells. Subsequently, the endogenous large subunit of RNA polymerase II was inhibited by alpha-amanitin and the induction of chromosomal c-fos and hsp70A genes was determined. Cells expressing the large subunit of RNA polymerase II and LSdelta31 were able to transcribe the c-fos and hsp70A genes after treatment with the phorbolester TPA and after heat-shock, respectively. In contrast, cells expressing LSdelta5 failed to induce expression of both genes.

References

Page 1

	Year	Citations
The C-terminal domain of RNA polymerase II couples mRNA processing to transcription Susan McCracken, Nova Fong, Krassimir Yankulov, Nature Transcriptional RegulationRna Polymerase IiNatural SciencesRna Binding ProteinsRna Biology	1997	905
The RNA polymerase II molecule at the 5′ end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged Ann E. Rougvie, John T. Lis Cell Transcriptional RegulationD. MelanogasterGeneticsUninduced Hsp70 GeneNatural Sciences	1988	666
5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II Susan McCracken, Nova Fong, Emanuel Rosonina, Genes & Development	1997	534
Identification of multiple cyclin subunits of human P-TEFb Jiexin Peng, Yuerong Zhu, John Milton, Genes & Development Systems BiologyProtein FunctionCmv PromoterMedicineGenetics	1998	518
Temporal Regulation of RNA Polymerase II by Srb10 and Kin28 Cyclin-Dependent Kinases Christoph J. Hengartner, Vic E. Myer, Sha-Mei Liao, Molecular Cell Signal TransductionRna Polymerase IiNatural SciencesMolecular RegulationMolecular Biology	1998	402
Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation Thomas O’Brien, S. E. Hardin, Arno L. Greenleaf, Nature Transcriptional RegulationNatural SciencesRna Binding ProteinsTranscriptional ElongationRna Biology	1994	326
A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. M. Mortillaro, Benjamin J. Blencowe, Xiumei Wei, Proceedings of the National Academy of Sciences Rna Polymerase IiGeneticsRna SplicingMolecular BiologyMolecular Genetics	1996	320
In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin Van Trung Nguyen Nucleic Acids Research	1996	262
The transition of RNA polymerase II from initiation to elongation is associated with phosphorylation of the carboxyl-terminal domain of subunit IIa John Payne, Paul J. Laybourn, Michael Dahmus Journal of Biological Chemistry Rna Polymerase IiSubunit IiaMolecular BiologyGene TranscriptionTranscriptional Regulation	1989	240
Phosphorylation of C-terminal domain of RNA polymerase II is not required in basal transcription Hiroaki Serizawa, Joan Conaway, Ronald Conaway Nature Basal TranscriptionTranscriptional RegulationRna Polymerase IiNatural SciencesRna Biology	1993	178

Page 1