Chromatographic separation of<i>cis</i> and<i>trans</i> fatty esters by argentation with a macroreticular exchange resin

Abstract

Abstract Methyl oleate and methyl elaidate, as well as other monoene cis and trans isomers of fatty esters, can be separated quickly and conveniently by a preparative chromatographic procedure in which a silver‐saturated ion‐exchange resin is used. Separations are based on differences in stabilites of the silver‐olefin complexes. Recoveries of better than 95% were made, and pure trans and cis monoene fractions were collected. This method can be used to separate saturates from cis and trans monoenes. The cis,trans,cis,cis , and trans,trans ‐9‐12‐octadecadienoates were separated. While cis,trans and trans,trans dienes were cluted separately, the cis,cis diene isomer remained on the column.