Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 1997 · 21 citations · 0 references
EngineeringBioengineeringBiomedical EngineeringBiocompatibilityBiomaterialsTissue EngineeringAnimal Cell CultureBiomaterials For Cell EngineeringCell CharacterizationCell DeliveryCell EncapsulationBioartificial OrgansCell Encapsulation MaterialsCell Viability PreservationCell-encapsulating MaterialsEncapsulation MaterialsEncapsulation StrategiesIslet Cell EncapsulationMammalian Cell Encapsulation
Gas phase silicon alkoxides react with the wet surface of mammalian cells, affording a stable and homogeneous layer of amorphous SiO<SUB>2</SUB> modified by Si-CH<SUB>3</SUB> and Si-H bonds. Layer thickness may be controlled by exposure time. The layer does not suppress cell viability or functionality, and may be applied to cells supported on a trapping network or to cell aggregates. H4-II-E-C3 rat hepatoma cells, Hep G2 human cancer cells and human fibroblasts on various supports were encapsulated by the SiO<SUB>2</SUB> layer and studied in terms of glucose utilization and <SUP>3</SUP>H-leucin incorporation into secreted proteins. In the case of pancreatic islets, encapsulation was carried out without supports, so that original islet volume and features were maintained. In vitro results indicate preservation of vitality and function, as tested by insulin production.