Angewandte Chemie International Edition · 2003 · 222 citations · 19 references
Life SciencesBiochemistryBiotechnologyBiophysical ChemistryBiosensorsEnzymologyBioanalytical ChemistryEnzymatic BiosensorsEnzyme-based BiosensorsNanomaterial-based BiosensorsProteolytic EnzymesProteolytic ProcessingHydrolytic EnzymesMagnetic BiosensorsMagnetic PropertiesMagnetic ResonanceProtease Activity AssaysProtease AssaysProteolytic ActivityProteolytic Enzyme Activity
Superparamagnetic nanosensors conjugated to avidin (CLIO-A) can be used to measure sensitively and quantitively the proteolytic cleavage of bi-biotinylated peptide substrates (rods in the scheme) by changes in magnetic resonance properties. This approach may be generalized to determine the activity of a wide range of hydrolytic enzymes.
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In vivo visualization of gene expression using magnetic resonance imaging
Angelique Y. Louie, Martina M. Hüber, Eric T. Ahrens et al. · Nature Biotechnology · 2000 · 1.1K citations
Magnetic relaxation switches capable of sensing molecular interactions
Juan Manuel Sánchez Pérez, Lee Josephson, T. O'Loughlin et al. · Nature Biotechnology · 2002 · 892 citations · Full text