Magnetic Sensors for Protease Assays

Abstract

Superparamagnetic nanosensors conjugated to avidin (CLIO-A) can be used to measure sensitively and quantitively the proteolytic cleavage of bi-biotinylated peptide substrates (rods in the scheme) by changes in magnetic resonance properties. This approach may be generalized to determine the activity of a wide range of hydrolytic enzymes.