An enzyme‐linked immunosorbent assay for total glycoalkaloids in potato tubers

Abstract

Abstract An antiserum raised against a solanine‐bovine serum albumin conjugate has been characterised and shown to be specific for the glycoalkaloids found in commercially available potatoes. The antiserum has been employed at high dilution in an enzyme‐linked immunosorbent assay (ELISA) for the glycoalkaloids. The assay is sensitive and precise and has many advantages over alternative procedures currently employed. Potato sample preparation is minimal and no recovery factors are required.