Proceedings of the National Academy of Sciences · 1992 · 151 citations · 40 references
Life SciencesDevelopmental BiologyImmunologyAdaptive ImmunityCellular ImmunologyDevelopmental ImmunologyAntigen PresentationAntigen RecognitionHistocompatibility AntigensMajor Histocompatibility ComplexT Cell BiologyT Cell DifferentiationAntigen-specific T Cell ResponsesCd8 Positive T CellsGamma Delta T Cell DifferentiationGamma-delta T Cell DevelopmentMarker Expression PatternsPositive SelectionPositive Selection MarkersT Cell Receptors
The specificity of T cells bearing gamma delta T-cell receptors (gamma delta+ T cells) is poorly characterized. Earlier studies suggest that like alpha beta+CD8+ T cells, some gamma delta+ T cells may recognize antigens associated with class I major histocompatibility complex molecules. alpha beta+CD8+ T cells are nearly absent in class I-deficient mice (mutant for beta 2-microglobulin), reflecting a requirement for intrathymic "positive selection" of these cells by class I molecules. Here, we examine whether the development of gamma delta+ T cells is altered in the beta 2-microglobulin mutant mice. We show that the cellularity, marker expression, repertoire, and functional competence of gamma delta+ T cells are not detectably deficient in beta 2-microglobulin mutant mice. We conclude that class I expression is unnecessary for the development of most gamma delta+ T cells.
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Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations