Journal of AOAC International · 2002 · 53 citations · 0 references
AgricultureBiotechnologyFood ScienceFood AnalysisFood SafetyTransgenic CropsCommercialization Of Transgenic CropsFood Safety AnalysisFood Safety AssessmentPolymerase Chain ReactionRegulatory Frameworks For Transgenic CropsAnalytical Result ReportingCompetitive Polymerase Chain ReactionGenetically Modified Food RegulationPolymerase Chain Reaction Product DetectionQuantitative Pcr DetectionReal-time Polymerase Chain Reaction (Pcr)Regulatory Assessment Of Transgenic CropsTransgenic Plants
Consequential to the implementation of European Commission (EC) Regulation 1139/98, EC Regulation 49/2000, and EC Regulation 50/2000 has been the need to measure accurately the levels of the genetically modified (GM) species Roundup Ready Soya and Bt 176 Maize that are present in food. Analytical methods to detect and quantitate these transgenic species have received much attention particularly with respect to the deminimus threshold of 1% for their presence in materials derived from non-GM identity-preserved (IP) supplies. The relative advantages and limitations of threshold analysis by double-competitive polymerase chain reaction (PCR) and quantitative real-time PCR are discussed in their application to the quantitative analysis of processed foods. Consideration is also given to other factors involved in the analyses that affect the performance of quantitative procedures, and to the many uncertainties involved in the precision of a reported analytical result.