Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2003 · 20 citations · 5 references
EngineeringBioengineeringBiomedical EngineeringBiomemsMicrofabricationMolecular DiagnosticsCancer Molecular DiagnosticsMicrofluidic Device FabricationMicrofluidic DevicesMicrofluidic SystemsMicrofluidicsPolymerase Chain ReactionBioanalytical ApplicationsBiomedical ApplicationsLiga ProcessMicrofluidic BioanalysisMicrofluidic Device DesignMicrofluidic Diagnostic DevicesMicrofluidic Diagnostic PlatformsPolymerase Chain Reaction (Pcr) Amplification
Two types of Microfluidic bioanalytical systems were designed and fabricated in polymer substrates using the LIGA process. A continuous flow polymerase chain reaction (CFPCR) Microfluidic device was fabricated in polycarbonate (PC), which utilized isothermal zone and shuttling the sample through each zone to achieve amplification. A 20-cycle PCR amplification of a fragment of a plasmid DNA template was achieved in 5.3 min. The results were comparable to those obtained in commercial laboratory-scale PCR system. The second system consisted of a microchip contating a low-density array assembled into the Microfluidic channel, which was hot-embossed in poly(methyl methacrylate) (PMMA). The detection of low-abundant mutations in gene fragments (<i>K-ras</i>) that carry point mutations with high diagnostic value for colorectal cancer was successfully performed. The array accessed microfluidics in order to enhance the kinetic associated with hybridization.
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Michael A. Innis, K B Myambo, David H. Gelfand et al. · Proceedings of the National Academy of Sciences · 1988 · 779 citations · Full text