Concepedia

Studies on Ghosting, an Important Source of Error In the Quantitative Estimation of Free Volatile Fatty Acids by GLC I. Occurrence of Ghosting and Factors Influencing It

Christian Van Eenaeme, Jean-Marie Bienfait, Olivier Lambot, André Pondant

Journal of Chromatographic Science · 1974 · 24 citations · 0 references

Concepts

AgricultureBiochemistryFood ScienceFood AnalysisFood ChemistryHuman NutritionChromatographic Food AnalysisFatty Acid ChemistryFatty Acid MetabolismLipid AnalysisVolatile Organic CompoundsChromatographic AssaysChromatographic QuantificationFatty AcidsVolatile Compounds AnalysisVolatile Fatty Acids

Abstract

Ghosting resulting from adsorption phenomena or surface reactions is an important source of error in quantitative determination of C2–C5 acids in gas liquid chromatography (GLC). The influence of several experimental variables on ghosting has been tested in a factorially designed experiment. Ghosting has been found to be dependent on column packing, acid nature, injector type, nature of the ghost eluter and injection sequence.