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A robust two-step PCR method of template DNA production for high-throughput cell-free protein synthesis

EngineeringMolecular Biological MethodNatural SciencesTemplate Dna ProductionDna AnalysisOligonucleotideMolecular BiologyDna ReplicationSynthetic BiologyNucleic Acid AmplificationProtein EngineeringDna ComputingReal-time Polymerase Chain ReactionGenome EditingBiomolecular Engineering