Plant Breeding · 2006 · 73 citations · 7 references
AgricultureGeneticsPlant SciencesCereal ScienceMolecular BreedingPlant GeneticsCereal BreedingCereal GeneticsCrop PhenotypingGene MappingGenetic MappingMolecular Markers In PlantsCereal Crop GeneticsCultivar CharacterizationCultivar DiscriminationGrain Processing QualityMolecular Marker BreedingPcr-based Marker DevelopmentPrimer ApplicationWheat Breeding
Abstract The 1BL·1RS translocation has been widely used in wheat breeding programmes throughout the world. Unfortunately, this translocation has frequently resulted in unsatisfactory grain processing quality. Two primer combinations derived from the published sequence of a ω ‐secalin gene on 1RS gave polymerase chain reaction (PCR) fragments 0.4 and 1.1 kb in size. Both fragments can be used to quickly detect 1BL·1RS translocations. By combining the PCR assay resulting in the 1.1‐kb fragment from 1RS and a PCR assay resulting in a 0.6‐kb fragment from the Glu‐B3 gene on 1BS, plants homozygous for the 1BL 1RS could clearly be distinguished from the heterozygous ones. This codominant marker was successfully applied to genotype a segregating F 2 population and a local cultivar collection.
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M. A. Saghai Maroof, K. M. Soliman, Richard A. Jorgensen et al. · Proceedings of the National Academy of Sciences · 1984 · 5.2K citations · Full text