Sensitive and rapid detection of<i>Shigella</i>and enteroinvasive<i>Escherichia coli</i>by a loop-mediated isothermal amplification method

Abstract

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.