Publication | Closed Access
Two-Dimensional Protein Separation by the HPLC System with a Monolithic Column
10
Citations
10
References
2012
Year
EngineeringTwo-dimensional Protein SeparationHplc SystemProtein Phase SeparationProtein SeparationProtein PurificationSeparation ScienceProtein FoldingBioanalysisCell SurfaceAnalytical ChemistrySeparation TechniqueLiquid ChromatographyProteomicsAdvanced SeparationBiophysicsChromatographyBiochemistryChromatographic AnalysisBiomolecular EngineeringIon-exchange ColumnMonolithic ColumnMass SpectrometryMedicine
A two-dimensional high-performance liquid chromatography (2D-HPLC) system for protein separation was developed using an ion-exchange column in the first dimension and a reversed-phase monolithic column in the second dimension. The system demonstrated efficient separation of proteins in comparison with conventional systems. For proteomic analysis, proteins extracted from the cell surface of the yeast were separated by 2D-HPLC and evaluated.
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