Journal of Interferon & Cytokine Research · 2000 · 11 citations · 38 references
Life SciencesCell BiologyMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationCytokine BiologyCytokine Receptor SignalingCytokine RegulationCytokine SignalingInflammatory SignalingInterferon ResponsesInterferon Gamma ResponseInterferon Signaling ResponsesInterferon-gamma ResponsesIntermediate Filament StructureJak-stat Signaling PathwayReporter Gene AssaysType Ii Interferon SignalingVimentin Expression
Vimentin is an intermediate filament protein normally expressed in cells of mesenchymal origin. Here, we report an increase in vimentin gene transcription induced by the cytokine interferon-y (IFN-gamma). Northern blot analysis and reporter gene assays reveal that IFN-gamma induces vimentin gene transcription in HeLa cells. However, no increase in vimentin mRNA synthesis was observed de novo in MCF-7 cells, which do not already express vimentin. Band shift analysis shows that the Stat1alpha protein mediates vimentin induction by IFN-gamma. A human mutant fibroblast cell line (U3A), which lacks Stat1alpha but expresses vimentin mRNA, yields no increase in vimentin mRNA levels on the addition of IFN-gamma. These results suggest that the induction of vimentin gene expression might be an important part of a complex cellular response to IFN-gamma.
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Crystal Structure of a Tyrosine Phosphorylated STAT-1 Dimer Bound to DNA
Uwe Vinkemeier, Yanxiang Zhao, David Jeruzalmi et al. · Cell · 1998 · 718 citations · Full text