Concepedia

Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris

Manuel Mansur, Cecilia Cabello, Lester Hern�ndez, Jos� Pa�s, Laura A. Varas, J. Vald�s, Yanet Terrero, Abdel Hidalgo, Liuba Plana, Vladimir Besada,

Biotechnology Letters · 2005 · 97 citations · 19 references

DOI

Concepts

References

19

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

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[12] High-efficiency transformation of yeast by electroporation

Daniel M. Becker, Leonard Guarente · Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 801 citations

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High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene

Jeff J. Clare, F. B. Rayment, S. Ballantine et al. · Nature Biotechnology · 1991 · 393 citations · Full text

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Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies

Jeffrey J. Clare, Michael A. Romanes, F. B. Rayment et al. · Gene · 1991 · 331 citations

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High-yield secretion of recombinant gelatins byPichia pastoris

Marc W. T. Werten, Tanja J. van den Bosch, Richèle D. Wind et al. · Yeast · 1999 · 258 citations · Full text

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