Nucleosome Cores Have Two Specific Binding Sites for Nonhistone Chromosomal Proteins HMG 14 and HMG 17

Abstract

The binding of HMG 14 (or 17) to nucleosome cores produces two major additional bands on nondenaturing polyacrylamide gels. Bound HMG 14 alters the relative densities of the end-labeled DNA fragment distribution produced by deoxyribonuclease I digestion of reconstructed poly(deoxyadenylate-deoxythymidylate) nucleosome cores. These results indicate nucleosome cores have two specific binding sites for HMG 14 (or 17).