Publication | Closed Access
Confocal microscopy with a volume holographic filter
100
Citations
6
References
1999
Year
Volume HologramHolographyFluorescence MicroscopyDepth ResponseEngineeringMicroscope Image ProcessingMicroscopyMicrofabricationModified Confocal MicroscopeMicroscopy MethodBiomedical ImagingHolographic MethodLight MicroscopyMedicineBiophysicsDigital Holography
We describe a modified confocal microscope in which depth discrimination results from matched filtering by a volume hologram instead of a pinhole filter. The depth resolution depends on the numerical aperture of the objective lens and the thickness of the hologram, and the dynamic range is determined by the diffraction efficiency. We calculate the depth response of the volume holographic confocal microscope, verify it experimentally, and present the scanned image of a silicon wafer with microfabricated surface structures.
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