Identification of Auxin from Pseudomonas sp. P7014 for the Rapid Growth of Pleurotus eryngii Mycelium

Abstract

Pseudomonas sp. P7014 박테리아를 통한 큰느타리버섯 균사체의 생육촉진에 관한 연구가 수행되었다. 박테리아 배양액으로 부터 분리한 ethyl acetate 분획물(F5)에는 성장촉진물질(GPC)이 함유되어 있음을 확인하였다. TLC, HPLC, NMR 및 MS/MS분석법으로 확인한 바, indole acetic acid (IAA)로 확인되었다. 큰느타리버섯 균사체는 성장촉진물질(GPC)이 첨가된 PDA와 PDB 배지에서 빠른 성장을 보였다. 성장촉진물질(GPC)의 농도는 1.0 nM로 매우 낮았지만, 확인된 tryptophan은 IAA의 전구체로써 IAA가 아민화된 형태였다. 이들 결과는 박테리아에서 분비된 성장촉진물질(GPC)은 IAA이었고 큰느타리버섯 균사체의 생육촉진에 중요한 역할을 하는 것으로 확인되었다. The promoting effect of Pseudomonas sp. P7014 on the mycelia growth of Pleurotus eryngii was investigated. An ethyl acetate fraction (F5) from the culture supernatant of the bacteria was confirmed to contain the growth promoting compound (GPC). The GPC was identified to be indole acetic acid (IAA) by TLC, HPLC, MS/MS, and NMR analyses. P. eryngii mycelia grew rapidly both on PDA and in PDB after the treatment of GPC. The promoting concentration of GPC was as low as 1.0 nM. Tryptophan, the aminated form of IAA, was confirmed to be the precursor of IAA. These results suggested that bacterial secreted compound was IAA and plays an important role in promoting growth of mushroom mycelia.