Nucleotide sequence from the neurogenic locus Notch implies a gene product that shares homology with proteins containing EGF-like repeats

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

A simple method for displaying the hydropathic character of a protein

Jack Kyte, Russell F. Doolittle · Journal of Molecular Biology · 1982 · 23.1K citations

Life Sciences, Biochemistry, Molecular Biology +12

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A comprehensive set of sequence analysis programs for the VAX

John Devereux, Paul Haeberli, Oliver Smithies · Nucleic Acids Research · 1984 · 14.4K citations · Full text

Life Sciences, Bioinformatics, Genetics +8

A general method applicable to the search for similarities in the amino acid sequence of two proteins

Saul B. Needleman, Christian D. Wunsch · Journal of Molecular Biology · 1970 · 11.5K citations

Life Sciences, Biochemistry, Bioinformatics +13