Bioscience Biotechnology and Biochemistry · 2001 · 41 citations · 16 references
Life SciencesChemistryMicrobiologyMetabolismNatural Product BiosynthesisOrganic ChemistryFungal BiochemistryFungal Natural Product BiosynthesisFungal Secondary MetabolitesMetabolite IdentificationMicrobial Metabolite ProductionMicrobial Natural Product BiosynthesisAntimicrobial MetabolitesBioactive Fungal MetabolitesFree Radical ScavengingFungal Bioactive CompoundsFungal Metabolite BiosynthesisIsolationRadical ScavengersStructure Elucidation Of Fungal Metabolites
In the course of our screening program for free radical scavengers from Trichoderma sp. USF-2690, we found an unidentified metabolite (1) that appeared by the method used for HPLC analysis. Metabolite 1 gradually decreased with the production of bisorbicillinoids and was easily missed during the general isolation procedure. The LC-ESI-MS (negative) analysis for 1 gave m/z 247 as the (M-1)- ion peak. The hydrolysis of synthetic 6-O-acetylsorbicillinol (+/- -2) by 0.05 M KOH and acetylation of product 1 in an aqueous solution indicated that the structure of 1 was (6S)-4-(2,4-hexadienoyl)-3,6-dihydroxy-2,6-dimethyl-2,4-cyclohexadien-1-one, designated sorbicillinol, a quinol that has been postulated to be important in bisorbicillinoid biosynthesis.
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Antioxidant Determinations by the Use of a Stable Free Radical
Marsden S. Blois · Nature · 1958 · 12.8K citations
K. C. Nicolaou, Georgios Vassilikogiannakis, Klaus B. Simonsen et al. · Journal of the American Chemical Society · 2000 · 126 citations