Science · 1991 · 1.3K citations · 26 references
Life SciencesBiochemistryMolecular BiologyCell SignalingHormone BiochemistrySignal TransductionCalcium EndocrinologyCalcium-binding ProteinsEndocrine Regulation Of CalciumHormone Binding ProteinsHormone ReceptorsParathyroid Gland PhysiologyAdenylate Cyclase RegulationAdenylate Cyclase SignalingAdenylyl Cyclase RegulationG-protein-coupled ReceptorsMembrane Binding DomainsMembrane Protein DomainsMembrane-spanning DomainsReceptor-g Protein Coupling
The complementary DNA encoding a 585-amino acid parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor with seven potential membrane-spanning domains was cloned by COS-7 expression using an opossum kidney cell complementary DNA (cDNA) library. The expressed receptor binds PTH and PTHrP with equal affinity, and both ligands equivalently stimulate adenylate cyclase. Striking homology with the calcitonin receptor and lack of homology with other G protein-linked receptors indicate that receptors for these calcium-regulating hormones are related and represent a new family.
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A comprehensive set of sequence analysis programs for the VAX
John Devereux, Paul Haeberli, Oliver Smithies · Nucleic Acids Research · 1984 · 14.4K citations · Full text