Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
Proceedings of the National Academy of Sciences · 1987 · 923 citations · 36 references
Cd2 AntigenHematologyImmunologyImmunodominanceCd2 CdnaBlood CellAutoimmunityT Cell ImmunityAntigen ProcessingCell TransplantationMolecular CloningCellular Immune ResponseImmunotherapyMedicineCell BiologyRapid Immunoselection ProcedureSurface Antigen
A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.
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