Effect of Temperature and Fermentation Time of Crude Cellulase Production by Trichoderma Reesei on Straw Substrate

Abstract

Research was conducted to determine the effect of temperature and fermentation time of crude cellulase production by Trichoderma reesei on straw substrate. The research was arranged in a randomized complete factorial design consisting of two factors. The first factor is the temperature of fermentation consists of two levels, 27 ̊C and 37 ̊C. The second factor is the time fermentation consists of three levels, namely 5 d, 7 d, and 9 d. The results showed that the temperature and fermentation period significantly affect the observed parameters on crude cellulase production by Trichoderma reesei. The optimal treatment combination to produce crude cellulase with maximum activity is at 27 ̊C and fermentation time of 9 d with an average value of cellulase activity (filter paperase) and soluble protein respectively 0.041 193 7 unit·mL–1 and 0.021 613 mg·mL–1.