Glycogen Detection by in Vivo ¹³C NMR: A Comparison of Proton Decoupling and Polarization Transfer

Abstract

The performance of gated proton decoupling and polarization transfer with respect to glycogen detection by 13C NMR was investigated. Experiments were performed on a 1.5-T whole-body scanner using a 13C surface coil in combination with a proton head coil. Spectra were acquired from a glycogen phantom and from the lower leg of a healthy volunteer using proton decoupling and the polarization transfer method SINEPT. The signal strength of the C1 resonance of glycogen was determined and compared to a reference spectrum acquired without any form of sensitivity enhancement. In the phantom experiment both decoupling and SINEPT produced a signal gain of 3.5. Under in vivo conditions, the signal gain was approximately 2.5 for both techniques. We conclude that decoupling and polarization transfer are equivalently useful techniques for glycogen detection.