Effects of modified alternate-day fasting regimens on adipocyte size, triglyceride metabolism, and plasma adiponectin levels in mice

Abstract

Calorie restriction (CR) affects adipocyte function and reduces body weight. However, the effects of alternate-day fasting (ADF) on adipose biology remain unclear. This study examined the effects of ADF and modified ADF regimens on adipocyte size, triglyceride (TG) metabolism, and adiponectin levels in relation to changes in body weight and adipose mass. Twenty-four male C57BL/6J mice were randomized for 4 weeks among 1) ADF-25% (25% CR on fast day, ad libitum on alternate day), 2) ADF-50% (50% CR on fast day), 3) CR on fast day), and body weight of mice of the and were of in ADF-50% and in and the to in ADF-50% and and in ADF-50% and adiponectin levels were modified ADF and ADF regimens adipocyte in body weight adipose weight in the Calorie restriction (CR) affects adipocyte function and reduces body weight. However, the effects of alternate-day fasting (ADF) on adipose biology remain unclear. This study examined the effects of ADF and modified ADF regimens on adipocyte size, triglyceride (TG) metabolism, and adiponectin levels in relation to changes in body weight and adipose mass. Twenty-four male C57BL/6J mice were randomized for 4 weeks among 1) ADF-25% (25% CR on fast day, ad libitum on alternate day), 2) ADF-50% (50% CR on fast day), 3) CR on fast day), and body weight of mice of the and were of in ADF-50% and in and the to in ADF-50% and and in ADF-50% and adiponectin levels were modified ADF and ADF regimens adipocyte in body weight adipose weight in the in body to changes in in the of adipose triglyceride (TG) to changes in reduces and to to and of in the the for and to of for and to to in and adipose to the of adipocyte and levels of and of adipose to for of to and adipocyte and the body and in of and effects and restriction of effects on and of of and CR to of adipocyte of restriction on size, and in in (25% in weeks in 4 weeks of CR in of in of CR in restriction to of adipocyte function in the to adiponectin levels to in to CR in and randomized and levels in and of restriction in CR alternate-day fasting ADF regimens of ad libitum for day), fast ADF of the effects of CR fasting for of and in of restriction on in in of ADF for CR in the ADF regimens modified of restriction on the fast ad libitum on the effects of ADF modified ADF regimens on adipose metabolism, adipocyte size, and adiponectin levels in to the of study to the effects of ADF and modified ADF regimens on adipocyte metabolism, adipocyte size, and adiponectin levels in adipose biology of body weight and C57BL/6J male mice were were and and on and were for and to and the of mice were randomized to of the ad libitum on the and on alternate the ad libitum on the and on the fast the ad libitum on the and fast on alternate the and the ad libitum of to the mice in the ADF-50% and ADF-25% on the fast on the of 4 and weight the of the of of and the 1) and were for of and in the and of mice were the of and the weeks of the study of body to the of body to ad libitum for the of the were weeks of and the of of and adipose and were and in of and were to the of the the for to the for 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