Sensitive and specific detection of proviral bovine leukemia virus by 5′ Taq nuclease PCR using a 3′ minor groove binder fluorogenic probe

Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Kenneth J. Livak, Samantha Flood, J Marmaro et al. · Genome Research · 1995 · 1.6K citations · Full text

Life Sciences, Genetics, Molecular Biology +13

[17] A rapid alkaline extraction method for the isolation of plasmid DNA

H.C. Birnboim · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 972 citations

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3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

Igor V. Kutyavin · Nucleic Acids Research · 2000 · 795 citations

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Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

Christian Drosten, Stephan Göttig, Stefan Schilling et al. · Journal of Clinical Microbiology · 2002 · 631 citations · Full text

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Development of a TaqMan® PCR assay with internal amplification control for the detection of African swine fever virus

Donald P. King, Scott M. Reid, Geoffrey H. Hutchings et al. · Journal of Virological Methods · 2003 · 539 citations

Veterinary Medicine, Infectious Diseases, Microbiology +16