Expression of the c- <i>fos</i> Gene and of an <i>fos</i> -Related Gene Is Stimulated by Platelet-Derived Growth Factor

TLDR

The PDGF‑inducible clone shares nucleotide sequence homology with the third exon of c‑fos, reflected in predicted amino acid similarity. The authors differentiate mouse c‑fos and the fos‑related gene transcripts using gel electrophoresis and S1 nuclease analysis. The PDGF‑inducible clone is homologous to c‑fos, cross‑hybridizes with the mouse v‑fos gene, and its expression is induced by PDGF and further enhanced by PDGF plus cycloheximide.

Abstract

Complementary DNA clones of genes induced by platelet-derived growth factor (PDGF) in BALB/c-3T3 cells were isolated; one such clone contains a domain having nucleotide sequence homology with the third exon of c-fos. This nucleotide sequence homology is reflected in the predicted amino acid sequences of the gene products. Under low stringency conditions, the mouse v-fos gene cross-hybridizes with the PDGF-inducible complementary DNA clone. However, the messenger RNA transcripts of mouse c-fos and the new fos-related gene can be distinguished by gel electrophoresis and by S1 nuclease analysis. Expression of the authentic c-fos gene is induced by PDGF and superinduced by the combination of PDGF and cycloheximide.