Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei

The Genome of the African Trypanosome <i>Trypanosoma brucei</i>

Matthew Berriman, Elodie Ghedin, Christiane Hertz‐Fowler et al. · Science · 2005 · 1.7K citations

Genetics, African Trypanosomes, Molecular Genetics +16

A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei

Elizabeth Wirtz, Simone Leal, Claudia M. Ochatt et al. · Molecular and Biochemical Parasitology · 1999 · 1.4K citations

Functional Genomics, African Trypanosomiasis, Trypanosoma Brucei +10

Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes

Michele Markstein, Chrysoula Pitsouli, Christians Villalta et al. · Nature Genetics · 2008 · 554 citations · Full text

Gypsy Retrovirus Insulator, Engineering, Reverse Genetics +13

Inducible Gene Expression in Trypanosomes Mediated by a Prokaryotic Repressor

Elizabeth Wirtz, Christine Clayton · Science · 1995 · 257 citations

Functional genomics in Trypanosoma brucei: A collection of vectors for the expression of tagged proteins from endogenous and ectopic gene loci

Steven Kelly, Jenny Reed, Susanne Krämer et al. · Molecular and Biochemical Parasitology · 2007 · 255 citations · Full text