Colorimetric method for free fatty acids in serum validated by comparison with gas chromatography.

Abstract

We compared results by a simplified colorimetric copper-soap method for serum free fatty acids (Clin, Chem. 19: 419, 1973, modified) with those by a gas--liquid chromatographic method. The modified method requires only 100 microliters of sample, its standard curve is linear from 0.1 to 4.0 mmol/L (reference interval for adults: 0.2 to 0.8 mmol/L), and it is suitable for use with newborns. Comparison with the gas--liquid chromatographic method (n = 51) over a wide concentration range gave a correlation coefficient of 0.989. Between-run CVs varied from 4 to 10%, analytical recoveries from 97 to 104%. Triglycerides, salicylates, hemoglobin, and anticoagulants (except citrate) do not interfere, but bilirubin and phospholipids give small positive interferences. The colorimetric assay is used, along with other routine tests, to assess lipid status and estimate kernicterus risk in neonatal intensive-care patients receiving lipid nutrition parenterally.