An efficient protocol for shoot organogenesis and plant regeneration of buckwheat (Fagopyrum esculentum Moench.)

Abstract

The establishment of a simple and efficient protocol for shoot organogenesis and plant regeneration from the lateral cotyledonary meristems of buckwheat (Fagopyrum esculentum Moench.) is reported. We investigated the effect of different concentrations of cytokinins (BAP, kinetin and TDZ) on the efficiency of shoot organogenesis in buckwheat. Treatment with BAP significantly induced shoot regeneration from cotyledon cultures of buckwheat. The highest number of shoots per explant (5.7) and shoot length (1.3 cm) were obtained on MS medium containing 4.0 mg/l BAP. The addition of the AgNO3 at an optimal concentration of 7 mg/l substantially improved the shoot regeneration frequency, which was around 30% more than in control, but declined at concentrations beyond the optimal one. The regenerated shoots (about 1 cm in length) displayed a normal morphology and could be easily rooted without any plant hormone treatment. The rooted plants were hardened and transferred to soil with a 72% survival rate, where they grew normally.