Intracytoplasmic Detection of Cytokines in Neonatal Lymphocytes and Monocytes by Flow Cytometry

Abstract

To the Editor: In a recent publication in BLOOD, Chalmers et al[1][1]presented data on cytokine production of lymphocytes at the single-cell level using three-color flow cytometry. A total of 6 blood samples have been investigated. Their main finding was the lower production of interleukin-2 (IL-2