Fatty acid composition of phospholipids from subcellular particles of rat liver

Abstract

The object of this work was to determine the fatty acid composition of the different phospho- lipids present in the mitochondria and microsomes of rat liver. Cl6ment, Haimovici & Le Breton (1955) obtained data for the polyenoic acids of the neutral fat and of the total phospholipids, by spectrophotometry after alkali-isomerization, and Cl6ment, Cl6ment & Le Breton (1956) gave further data on the nature of the lipids. It appeared of interest to obtain more detailed information by preliminary fractionation of the lipids, and analysis of their fatty acids by gas chromatography. Part of this work has been briefly reported (Macfarlane, Gray & Wheeldon, 1960).