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Further Study on Gas Phase Acid Hydrolysis of Protein: Improvement of Recoveries for Tryptophan, Tyrosine, and Methionine
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1990
Year
Protein ChemistryBiochemistryNatural SciencesM HclBioanalysisQuantitative AnalysisEnzyme CatalysisMolecular BiologyProtein RefoldingCommon Amino AcidsStructure-function Enzyme KineticsMetabolismMedicineEnzymatic ModificationBiomolecular EngineeringChromatographyProtein Purification
A novel method for vapor phase acid hydrolysis of protein suitable for quantitative analysis of tryptophan is presented. The hydrolysis is carried out in vapor of a mixture made of 7 M HCl, 10% trifluoroacetic acid, and 20% thioglycolic acid in the presence of indole. Reasonably good recoveries of common amino acids, including tryptophan (above 75%), were achieved.