Cytochrome P-450scc-phospholipid interactions. Evidence for a cardiolipin binding site and thermodynamics of enzyme interactions with cardiolipin, cholesterol, and adrenodoxin.

Abstract

function as an electron shuttle (3-5); adre- nodoxin in its complex with NADPH-reduced reductase fist accepts an electron from the reduced flavoprotein, then dis- sociates and forms a 1:l complex with cytochrome P-450, and finally transfers an electron to the hemoprotein. Cholesterol binds to the cytochrome via the hydrophobic membrane in which this substrate