Identification of the mannosyl donors involved in the synthesis of lipid-linked oligosaccharides.

Abstract

The abbreviations used are: GlcNAc, N-acetylglucosamine; GlcitolNAc, N-acetylglucosaminitol; dolichol-P-mannose, dolichol monophosphorylmannose; Thy-I E, mutant mouse lymphoma cell line BW5147.3.10, which does not express the Thy-1 antigen.100 mM a-methylglucoside (at B).The open circles indicate radioactivity while the closed symbols indicate .'Hradioactivity.Fractions (0.5 ml) were pooled as shown by the bars and termed V'a and V'b.