The Amino Acid Sequence of Δ5-3-Ketosteroid Isomerase of Pseudomonas testosteroni

Abstract

Abstract The primary structure of the identical subunits of crystalline Δ5-3-ketosteroid isomerase (EC 5.3.3.1) of Pseudomonas testosteroni has been determined by standard procedures to be the following: [see PDF for sequence] Thus, each subunit consists of a single polypeptide chain of 125 amino acid residues. The subunit molecular weight, calculated from the amino acid sequence, is 13,394 or 33% of the ultracentrifugally determined molecular weight of the intact isomerase.