Purification of Ovalbumin Messenger Ribonucleic Acid by Specific Immunoadsorption of Ovalbumin-synthesizing Polysomes and Millipore Partition of Ribonucleic Acid

Abstract

Ovalbumin-synthesizing polysomes were isolated from total oviduct polysomes by reaction with anti-ovalbumin antibody and adsorption to a matrix of glutaraldehyde-linked ovalbumin. The RNA from immunoadsorbed ovalbumin polysomes was then deproteinized and selectively adsorbed to Millipore filters. Several types of experiments indicated the resulting RNA fraction is highly enriched for ovalbumin mRNA.