Mannanase Components from <i>Bacillus pumilus</i>

Abstract

beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH optima of 5.5 to 6.9 (A) and 6.0 (B); and half-lives of 60 (A) and 21 (B) min at 70 degrees C.