Theranostics · 2015 · 131 citations · 18 references
MedicineMolecular BiologyOncologyMolecular DiagnosticsMolecular ImagingMolecular OncologyCancer Molecular DiagnosticsLigand BindingMolecular Imaging In OncologyPancreatic CancerPancreatic PathologyTumor ImagingAptamer ChemistryAptamer DesignAptamer DiscoveryAptamer RecognitionAptamer-based ProbesAptamersCell-selexNucleic Acid Aptamer Selection
In this work, we have developed a truncated DNA aptamer, termed XQ-2d, with high affinity and specificity for pancreatic ductal adenocarcinoma (PDAC). Aptamer XQ-2d selectively binds to PL45 cells with a dissociation constant in the nanomolar range, as determined by its recognition of PL45 tumor cells in mice. Moreover, XQ-2d shows better recognition ratio for 40 tissue sections of clinical PDAC samples (82.5%) compared to the initial cell-SELEX selection library (5%). Therefore, XQ-2d can be considered a promising candidate as a tool for PDAC diagnosis and treatment.
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In vitro selection of RNA molecules that bind specific ligands
Andrew D. Ellington, Jack W. Szostak · Nature · 1990 · 9.7K citations
Development of DNA aptamers using Cell-SELEX
Kwame Sefah, Dihua Shangguan, Xiangling Xiong et al. · Nature Protocols · 2010 · 909 citations